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human il-2 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd human il-2 elisa kit
    Human Il 2 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+2+elisa+kit/Human+IL-2+ELISA+Kit/custom%40ek102%4042628379
    Average 95 stars, based on 108 article reviews
    human il-2 elisa kit - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Lactate Dehydrogenase Assay:

    Article Title: Interleukin 21-Armed EGFR-VHH-CAR-T Cell Therapy for the Treatment of Esophageal Squamous Cell Carcinoma
    Article Snippet: The Cytotoxicity LDH Assay Kit-WST (DOJINDO, Shanghai, China) was used to detect the LDH secretion of KYSE cell lines that were co-cultured with CAR-T cells or UTD T cells at different ratios (T cells: target cells = 2:1, 4:1, 8:1, 16:1, and 32:1). .. After 16 h, the culture supernatants were collected and detected by using an LDH release assay, a human IFN-γ ELISA kit (MultiSciences, Hangzhou, China), a human TNF-α ELISA kit (MultiSciences), a human IL-2 ELISA kit (MultiSciences), and a human IL-10 ELISA kit (MultiSciences), respectively. ..

    Article Title: Interleukin 21-Armed EGFR-VHH-CAR-T Cell Therapy for the Treatment of Esophageal Squamous Cell Carcinoma.
    Article Snippet: The Cytotoxicity LDH Assay Kit-WST (DOJINDO, Shanghai, China) was used to detect the LDH secretion of KYSE cell lines that were co-cultured with CAR-T cells or UTD T cells at different ratios (T cells: target cells = 2:1, 4:1, 8:1, 16:1, and 32:1). .. After 16 h, the culture supernatants were collected and detected by using an LDH release assay, a human IFN-γ ELISA kit (MultiSciences, Hangzhou, China), a human TNF-α ELISA kit (MultiSciences), a human IL-2 ELISA kit (MultiSciences), and a human IL-10 ELISA kit (MultiSciences), respectively. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Interleukin 21-Armed EGFR-VHH-CAR-T Cell Therapy for the Treatment of Esophageal Squamous Cell Carcinoma
    Article Snippet: The Cytotoxicity LDH Assay Kit-WST (DOJINDO, Shanghai, China) was used to detect the LDH secretion of KYSE cell lines that were co-cultured with CAR-T cells or UTD T cells at different ratios (T cells: target cells = 2:1, 4:1, 8:1, 16:1, and 32:1). .. After 16 h, the culture supernatants were collected and detected by using an LDH release assay, a human IFN-γ ELISA kit (MultiSciences, Hangzhou, China), a human TNF-α ELISA kit (MultiSciences), a human IL-2 ELISA kit (MultiSciences), and a human IL-10 ELISA kit (MultiSciences), respectively. ..

    Article Title: TNFSF10 drives hyperactive immune responses via NLRP3 inflammasome and endoplasmic reticulum stress in autoimmune and inflammatory diseases.
    Article Snippet: .. The kits included the QuantiCyto Human IFN-b ELISA kit (Cat. #: EHC026b.48, neobioscience), the QuantiCyto Human IL-29(IFN-k) ELISA kit (Cat. #: EHC015.48, neobioscience), the QuantiCyto Human IFN-a ELISA kit (Cat. #: EHC144a.48, neobioscience), the Human OPG ELISA Kit (Cat. #: ELK1176, ELK Biotechnology), the Human IL-6 ELISA Kit (Cat. #: EK106, Multi Sciences), the Human IL-2 ELISA Kit (Cat. #: EK102, Multi Sciences), the Human TNF-a High Sensitivity ELISA Kit (Cat. #: EK182HS, Multi Sciences), the Human IFN-c High Sensitivity ELISA Kit (Cat. #: EK180HS, Multi Sciences), and the Human TNFSF10 ELISA Kit (Cat. #: ELK1320, ELK Biotechnology). ..

    Article Title: Modification of PLAC8 by UFM1 affects tumorous proliferation and immune response by impacting PD-L1 levels in triple-negative breast cancer.
    Article Snippet: .. The supernatants were collected after 48 hours and examined by Human IL- 2 ELISA kit (#70- EK102- 96, MultiScience) according to the manufacturer’s instruction. ..

    Article Title: DNA-Templated Anchoring of Proteins for Programmable Cell Functionalization and Immunological Response.
    Article Snippet: CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit was purchased from Promega. .. Human TNF-α ELISA Kit, Human IL-2 ELISA Kit and Human IFN-γ ELISA Kit were bought from Lianke Biotech. ..

    Article Title: A programmable protease-based protein secretion platform for therapeutic applications.
    Article Snippet: Cell-based therapies represent potent enabling technologies in biomedical science.. However, current genetic control systems for engineered-cell therapies are predominantly based on the transcription or translation of therapeutic outputs.. Here we report a protease-based rapid protein secretion system (PASS) that regulates the secretion of pretranslated proteins retained in the endoplasmic reticulum (ER) owing to an ER-retrieval signal.

    Article Title: METTL14/IGF2BP2-mediated m6A modification of PD-L1 promotes proliferation, metastasis, and immune escape in high-grade gliomas.
    Article Snippet: N6-methyladenosine (m6A) RNA modification is involved in regulating the malignant progression and immune escape of glioblastoma multiforme (GBM).. This study investigated the role of methyltransferase-like protein 14 (METTL14), the central component of the m6A methylated transferase complex, in GBM progression and immune escape.. METTL14 and programmed death ligand 1 (PD-L1) levels were analyzed by qRT-PCR and Western blot in human GBM samples.

    Article Title: Validation of CCL20-driven CAR-γδ T secreting PD-1 blockade with enhanced trafficking into solid tumor
    Article Snippet: LEGEND MAX Human CCL20 ELISA Kit , Biolegend , Cat#: 441407. .. Human IL-2 ELISA Kit , MultiSciences , Cat#: 70-EK102-96. .. Human IFN-γ ELISA Kit , MultiSciences , Cat#: 70-EK180-96.

    Article Title: Interleukin 21-Armed EGFR-VHH-CAR-T Cell Therapy for the Treatment of Esophageal Squamous Cell Carcinoma.
    Article Snippet: The Cytotoxicity LDH Assay Kit-WST (DOJINDO, Shanghai, China) was used to detect the LDH secretion of KYSE cell lines that were co-cultured with CAR-T cells or UTD T cells at different ratios (T cells: target cells = 2:1, 4:1, 8:1, 16:1, and 32:1). .. After 16 h, the culture supernatants were collected and detected by using an LDH release assay, a human IFN-γ ELISA kit (MultiSciences, Hangzhou, China), a human TNF-α ELISA kit (MultiSciences), a human IL-2 ELISA kit (MultiSciences), and a human IL-10 ELISA kit (MultiSciences), respectively. ..

    Transduction:

    Article Title: A programmable protease-based protein secretion platform for therapeutic applications.
    Article Snippet: Cell-based therapies represent potent enabling technologies in biomedical science.. However, current genetic control systems for engineered-cell therapies are predominantly based on the transcription or translation of therapeutic outputs.. Here we report a protease-based rapid protein secretion system (PASS) that regulates the secretion of pretranslated proteins retained in the endoplasmic reticulum (ER) owing to an ER-retrieval signal.



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    Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels <t>of</t> <t>IL-4</t> (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.
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    The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin <t>ELISA</t> assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.
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    The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin <t>ELISA</t> assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.
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    The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin <t>ELISA</t> assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.
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    Image Search Results


    Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels of IL-4 (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.

    Journal: Frontiers in Physiology

    Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

    doi: 10.3389/fphys.2026.1906184

    Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels of IL-4 (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.

    Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

    Techniques: Expressing

    Effects of IL-13 130R and IL-13 130Q on human bronchial smooth muscle cell (hBSMC) phenotypes (n = 4 per group). (A) CCK-8-derived proliferative/metabolic activity. (B) Representative Fluo-3/AM flow-cytometry histograms and median fluorescence intensity quantification of the acetylcholine-induced intracellular calcium response. (C, D) IL-5 (C) and IL-4 (D) concentrations in culture supernatants. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; SEM, standard error of the mean.

    Journal: Frontiers in Physiology

    Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

    doi: 10.3389/fphys.2026.1906184

    Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on human bronchial smooth muscle cell (hBSMC) phenotypes (n = 4 per group). (A) CCK-8-derived proliferative/metabolic activity. (B) Representative Fluo-3/AM flow-cytometry histograms and median fluorescence intensity quantification of the acetylcholine-induced intracellular calcium response. (C, D) IL-5 (C) and IL-4 (D) concentrations in culture supernatants. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; SEM, standard error of the mean.

    Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

    Techniques: CCK-8 Assay, Derivative Assay, Activity Assay, Flow Cytometry, Fluorescence

    The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin ELISA assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.

    Journal: bioRxiv

    Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

    doi: 10.64898/2026.08.20.746041

    Figure Lengend Snippet: The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin ELISA assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.

    Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

    Elabela (ELA) or apelin treatment decreased IL-6 mRNA in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=3-6.

    Journal: bioRxiv

    Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

    doi: 10.64898/2026.08.20.746041

    Figure Lengend Snippet: Elabela (ELA) or apelin treatment decreased IL-6 mRNA in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=3-6.

    Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

    Techniques:

    Schematic of proposed interactions between IL-6 signaling and apelin and elabela in HTR-8/SVneo trophoblastic cells under CoCl₂-mimicked hypoxia. We hypothesize that apelin/elabela/APJ axis regulates IL-6 signaling via its actions on various components of the IL-6 classical and trans-signaling pathway.

    Journal: bioRxiv

    Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

    doi: 10.64898/2026.08.20.746041

    Figure Lengend Snippet: Schematic of proposed interactions between IL-6 signaling and apelin and elabela in HTR-8/SVneo trophoblastic cells under CoCl₂-mimicked hypoxia. We hypothesize that apelin/elabela/APJ axis regulates IL-6 signaling via its actions on various components of the IL-6 classical and trans-signaling pathway.

    Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

    Techniques: